Biotin is an unnumbered member of the B-complex family, normally only required in minute amounts. Biotin, a water-soluble vitamin, is used as co-factor of enzymes involved in fatty acid metabolism, gluconeogenesis, and amino acid catabolism, making biotin essential in maintaining metabolic homeostasis. Biotin plays an important role in metabolic functioning as a coenzyme carrier of activated carbon dioxide in the TCA cycle (also known as the Krebs cycle).237 In its coenzyme form, biotin synthesizes glucose from noncarbohydrate sources, and synthesizes and breaks down certain fatty acids and amino acids.213
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